Reporter

Part:BBa_K1997004:Design

Designed by: Xinyuan Qiu   Group: iGEM16_NUDT_CHINA   (2016-10-13)


sGFP-N->Zif268->sGFP-C


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 688
    Illegal AgeI site found at 772
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 775
    Illegal BsaI.rc site found at 508
    Illegal BsaI.rc site found at 989


Design Notes

This part is codon optimized for expression in E.coli This part is codon optimized for expression in E.coli, a 10aa linker was added to the both sides of the cds.



Source

This part is custom synthesized, the original sequence was acquired form fire fly

References