Composite
Part:BBa_K1994022:Design
Designed by: Isobel Holden Group: iGEM16_Warwick (2016-10-14)
Multiple dCas9 binding sites with weak omega promoter upstream of GFP + dCas9 expression cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 225
Illegal NheI site found at 331
Illegal NheI site found at 2353 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 248
Illegal BamHI site found at 4632
Illegal XhoI site found at 361 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 229
Illegal AgeI site found at 397 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
dCas9 with Pam staggered sequence and omega promoter
Source
dCas9 with Pam staggered sequence and omega promoter