RNA

Part:BBa_K1994013

Designed by: Liam Carroll   Group: iGEM16_Warwick   (2016-09-22)


sgRNA with dCas9 binding site sequence 9 and PP7 handle insert

For use in CRISPR/Cas9 systems. Contains golden gate adapter at 5' end for inserting target sequence. Also contains second golden gate adapter between truncated terminator and aspA terminator. Both golden gate adapters contain Bsa1 cut sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/crispr
//function/crispr/cas9
Parameters
None