Composite

Part:BBa_K1992011:Design

Designed by: Shiran Sarig, Inbal Adir   Group: iGEM16_Technion_Israel   (2016-10-13)

Histamin-Tar expression system(promoter+RBS+coding+terminator)

Novel Histamine-Tar chemoreceptor in E.coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 184


Design Notes

Primers used for inserting point mutations at region I:
Forward: 5'-TCA GCG GTA CGG ATG ATG ATG GA- 3'
Reverse: 5'-ACG CCA CAG GTT AAT TTC CGT TTG CAG CAT- 3'
Primers used for inserting point mutations region II:
Forward: 5'-ACC TGG GGA ATG ATG AAT GCA ATG GGC- 3'
Reverse: 5'-TGG ATG AGC CAC TTC AGC TCC AGT ATT- 3'