Composite

Part:BBa_K1992010:Design

Designed by: Shilo Ohayon   Group: iGEM16_Technion_Israel   (2016-10-14)


PctA-Tar GFP tagged expression system (promoter+RBS+coding+terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 331
    Illegal NgoMIV site found at 412
    Illegal NgoMIV site found at 416
    Illegal NgoMIV site found at 435
    Illegal AgeI site found at 535
    Illegal AgeI site found at 746
    Illegal AgeI site found at 1601
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2657


Design Notes

none


Source

none

References