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Part:BBa_K199199:Design

Designed by: Mary Gearing   Group: iGEM09_MoWestern_Davidson   (2010-12-12)


ATG-loxP-STOP sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 51


Design Notes

Designed to include multiple stop codons in all 6 reading frames.


Source

loxP is J61042 Sequence also contains bidirectional transcriptional terminator B0014.

References