Part:BBa_K199193:Design

TT-pLux-RBS-LuxI 2 sites-RBS-RFP-RBS-GFP-pBAD-RBS-LuxR

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 483
  Illegal AgeI site found at 1426
  Illegal AgeI site found at 1538
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 135
  Illegal BsaI.rc site found at 2250
  

Design Notes

Introduction of restriction sites for AscI and AsiSI required changing several amino acids and retaining the reading frame.

Source

Registry intermediates