Device

Part:BBa_K199095

Designed by: Mary Gearing   Group: iGEM09_MoWestern_Davidson   (2009-10-18)

pLac-RBS-CCACU-RFP-pTet-CCACU tRNA

Reporter construct ligated upstream of a suppressor tRNA. The tRNA suppresses the 5 bp insertion in the RFP gene.

This device works twice as well as its sister device of Part:BBa_K199094.

See Part:BBa_K199095:Experience link for characterization of this device.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1008
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 786
    Illegal AgeI site found at 898
    Illegal AgeI site found at 1045
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apLac-RBS-CCACU-RFP-pTet-CCACU tRNA