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Part:BBa_K199075:Design

Designed by: Clifton Davis   Group: iGEM09_MoWestern_Davidson   (2009-09-20)


RBS RFP with CUACC Addition


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Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 578
    Illegal AgeI site found at 690
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

The template of the gene comes from Part BBa_E1010. We designed a forward primer that included RBS,ATG, the 5-bp addition CTACC and the first 20 nucleotides on the 5' end of RFP after ATG. This made the edited 5' sequence for our RFP.

References