Composite

Part:BBa_K199056:Design

Designed by: Olivia Ho-Shing   Group: iGEM09_MoWestern_Davidson   (2009-08-05)

CCAUC tRNA (9-bp Anticodon) with RFP Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 176
    Illegal AgeI site found at 1075
    Illegal AgeI site found at 1187
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See the following parts for more information: CCAUC tRNA, pBad-tRNA and RFP with 5-bp addition. We wanted to create this composite so that the tRNA and reporter would be replicated in the same plasmid, and to be more efficient than a double transformation.

Source

From the BioBrick Parts Registry. The tRNA was constructed by oligo assembly, and mutated the RFP reporter, E1010, to add the 5-bp insertion by PCR.

References