Coding

Part:BBa_K1988013:Design

Designed by: Bowman Clark   Group: iGEM16_Purdue   (2016-10-14)


his-tagged PPK1 homologue w/ Anderson Promoter J23106


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 378


Design Notes

Codon optimized for E. coli K-12 using IDT Codon Optimizer


Source

Genetic sequence from 'Deciphering the Genome of Polyphosphate Accumulating Actinobacterium Microlunatus Phosphovorus' by Kawakoshi, et. al.

References