Coding
Part:BBa_K1988004:Design
Designed by: Bowman Clark Group: iGEM16_Purdue (2016-10-14)
Pit B: Inorganic P Transport from M. Phosphovorus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 982
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1186
Design Notes
Codon optimized for E. coli K-12 using IDT Codon Optimizer
Source
Genetic sequence from 'Deciphering the Genome of Polyphosphate Accumulating Actinobacterium Microlunatus Phosphovorus' by Kawakoshi, et. al.