Coding

Part:BBa_K1985007:Design

Designed by: Rita Adriani, Jasmine Cornish   Group: iGEM16_Kent   (2016-10-14)


AraC pBAD mamO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2081
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1946
    Illegal AgeI site found at 2030
    Illegal AgeI site found at 2939
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2561
    Illegal BsaI.rc site found at 1232
    Illegal SapI site found at 961


Design Notes

Codon Optimised


Source

The part comes from Magnetospirillum gryphiswaldense, sourced from Rokas Juodeikis in Professor Martin Warren's lab at the University of Kent.


References