![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1983013:Design
Designed by: Vykintas Jauniškis Group: iGEM16_Vilnius-Lithuania (2016-10-13)
Phenylalanine-specific permease (PheP) from Escherichia coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 37
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers
Primers used for amplification of the fragment:
Uni-FW: GTAGAATTCGCGGCCGCTTCTAG
Uni-RV: GTAGACTGCAGCGGCCGCTACTAG
Primers used for colony PCR screening:
For pSB1C3:
VF2: tgccacctgacgtctaagaa
VR: attaccgcctttgagtgagc
Source
This part is derived from Escherichia coli and was synthesized by Integrate DNA Technologies.