Coding

Part:BBa_K1982009:Design

Designed by: Zexu Li   Group: iGEM16_NEU-China   (2016-10-09)


Eukaryotic Cryptochrome 2 (CRY2) ( a blue light stimulated photoreceptor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 405
    Illegal BglII site found at 864
    Illegal BamHI site found at 1343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 289
    Illegal AgeI site found at 1018
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 641
    Illegal BsaI.rc site found at 50
    Illegal SapI.rc site found at 158


Design Notes

mutant EcoR 1 : TGTTGGGAATTc--->TGTTGGGAATTg


Source

The light-inducible heterodimerizering proteins CRY2 and CIB1 are both from Arabidopsis thaliana.

References