Part:BBa_K1978002:Design

TorA-RFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 691
  Illegal AgeI site found at 803
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The design of the linker was crucial, as well as, the codon optimization of TorA.

Source

TorA was synthesized by IDT but the basic amino acid sequence comes from E. coli. The RFP used stems from another iGEM vector.