Composite

Part:BBa_K1978001:Design

Designed by: Larissa Krüger   Group: iGEM16_Goettingen   (2016-10-13)


TorA-GlmS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequences were designed by codon optimization and exclusion of restriction sites present in iGEM prefix and suffix. The linker needed to be constructed in a way that is similar to the GlmS amino acid sequence.


Source

All DNA sequences were synthesized by IDT on the basis of the amino acid sequence of TorA in E.coli and GlmS from Clostridium cochlearium.

References