Coding

Part:BBa_K1975003:Design

Designed by: Joachim Steen Larsen   Group: iGEM16_UNIK_Copenhagen   (2016-06-28)


PhaB - NADPH-dependent acetoacetyl-CoA reductase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To secure the most optimal expression in Bacillus subtilis as possible, we decided to get the sequence codon optimized by using the Codon optimization tool from IDT.


Source

genetic sequence for PhaB is original from Ralstonia eutropha genomic DNA, but has been optimized to the expression of B. subtilis

References

Taguchi et. al. 2008: "A microbial factory for lactate-based polyesters using a lactate-polymerizing enzyme", PNAS

Yamada et. al. 2010: "Adjustable Mutations in Lactate (LA)-Polymerizing Enzyme for the Microbial Production of LA-Based Polyesters with Tailor-Made Monomer Composition", Biomacromolecules