Coding

Part:BBa_K1975002:Design

Designed by: Joachim Steen Larsen   Group: iGEM16_UNIK_Copenhagen   (2016-06-28)


PhaA - Beta-kethothiolase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To secure the best expression as possible, we decided to get the sequence codon optimized. This was done using the Codon optimization tool from IDT.


Source

This sequence origin from Ralstonia eutropha, but it has been codon optimized for Bacillus subtilis.

References

Taguchi et. al. 2008: "A microbial factory for lactate-based polyesters using a lactate-polymerizing enzyme", PNAS

Yamada et. al. 2010: "Adjustable Mutations in Lactate (LA)-Polymerizing Enzyme for the Microbial Production of LA-Based Polyesters with Tailor-Made Monomer Composition", Biomacromolecules