Coding

Part:BBa_K1975000:Design

Designed by: Joachim Steen Larsen   Group: iGEM16_UNIK_Copenhagen   (2016-06-28)


LPE - Lactate polymerizing enzyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 445


Design Notes

To secure as optimal expression as possible, the sequence has been codon optimized for Bacillus subtilis by using the Codon optimization tool from IDT.


Source

This enzyme originate from Pseudomaonas Sp. 61-3, however it has later been mutated to alter its specificity.

References