![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1973018:Design
Designed by: Alvaro Escobar Doncel Group: iGEM16_UPO-Sevilla (2016-10-12)
nahR-Psal-nasF-pleD*
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
Illegal BamHI site found at 1188
Illegal BamHI site found at 2345 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 408
Illegal NgoMIV site found at 1603
Illegal NgoMIV site found at 1750
Illegal AgeI site found at 2086 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1261
Design Notes
We digested BBa_K1973016 as a vector (EcoRI and XbaI) and BBa_K1973005 as an insert (EcoRI and SpeI), and then we ligate them.
Source
Molecular cloning.