Intermediate
Part:BBa_K1973005:Design
Designed by: Alvaro Escobar Doncel Group: iGEM16_UPO-Sevilla (2016-10-11)
nahR-Psal-nasF
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 408
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We digested BBa_K1973001 as a vector (EcoRI and XbaI) and BBa_K1973033 as an insert (EcoRI and SpeI), and then we ligate them.
Source
Molecular cloning.