Coding

Part:BBa_K1972002:Design

Designed by: Shuli Liang   Group: iGEM16_SCUT-China_A   (2016-09-16)


dszC (a monooxygenase gene )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 98
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 902
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1052


Design Notes

DszC require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) while working.


Source

The monooxygenase was found in Rhodococcus erythropolis IGTS8 and the gene was synthesized and the sequence was found in NCBI (GenBank Accession number L37363.1).

References