DNA

Part:BBa_K1971002:Design

Designed by: KE GUAN YU   Group: iGEM16_BHU_China   (2016-10-20)


crm197 gene with BsaI site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2
    Illegal BsaI.rc site found at 1633
    Illegal SapI.rc site found at 125


Design Notes

it has to be optimized in order to eliminate the EcorI site


Source

From artificial sequence

References