Coding

Part:BBa_K197009:Design

Designed by: Gabriela Guzman Lopez Aguado   Group: iGEM09_Berkeley_Wetlab   (2009-10-21)

{espP(beta)}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 690
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]

Source

PCR ca998/O09ig280 on M10044		  	(1362, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5           	(EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-B09espP   		{<espP(beta)>}
----
ca998	Forward Oligo 
CCATAGAATTCATGAGATCTTGGACCCAGATGTCTTCTGGCACTC
O09ig280	Reverse Oligo for the removal of the stop codon from {<espP(beta)>}
CGTTAGGATCCgaacgagtaacggaaattagcg

References