Composite

Part:BBa_K1969022:Design

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-12)


ADRB2 with His-tag regulated by GAL1 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 453
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 453
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1261
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 453
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 453
    Illegal AgeI site found at 70
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 847
    Illegal BsaI.rc site found at 1045


Design Notes

The His-tag is used to monitor the expression and membrane localization of ADRB2 gene.


Source

Combination of corresponding basic parts.

References