Regulatory

Part:BBa_K1969005:Design

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-08)


CUP1 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 64
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 64
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As the promoter sequence is rather short, we may use recombinase to inset it upstream different coding sequence to control the expression level.


Source

The genomic sequence from Saccharomyces cerevisiae.

References