Regulatory

Part:BBa_K1969003

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-05)


Full length S. cerevisiae ADH1 promoter

This is the full length of yeast ADH1 promoter cloned from genomic DNA of Saccharomyces cerevisiae. Although originally thought to be expressed constitutively, ADH1 promoter mediated ADH1 transcription is repressed when cells are grown on a non-fermentable carbon source such as ethanol or glycerol. Thus when culturing yeast with non-fermentable medium, researchers should pay attention to the expression level.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 90


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Categories
Parameters
None