Reporter

Part:BBa_K1968023:Design

Designed by: Ricardo Camilo Chávez Martínez   Group: iGEM16_Edinburgh_OG   (2016-10-14)


weLOV2 Fluorescent protein Phytobrick


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coding sequences can sometimes be troublesome to work with, especially if these are not codon optimized for the selected organism. Since the team was going to work with four different organisms (E. coli, Rhodococcus justt, Penicillium roqueforti, Synechocystis sp. PCC6803) as an alternative to synthesizing the optimized version for each organism a different approach was discussed and implemented.

Designed under the Phytobrick standard.



Source

Based ont he ilov sequence from BBa_K660004.

This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing

References