Coding

Part:BBa_K1965022:Design

Designed by: Katja Leben   Group: iGEM16_Slovenia   (2016-10-17)


nLuc:AP4:TEVs:P3mS-2A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1723
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 103
    Illegal NgoMIV site found at 1447
    Illegal NgoMIV site found at 1468
    Illegal AgeI site found at 1171
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1538
    Illegal SapI site found at 1694
    Illegal SapI.rc site found at 1353


Design Notes

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Source

nLuc -Photinus pyralis (Common eastern firefly)
AP4, P3ms-2A - artificial sequence

References