Coding
Part:BBa_K1964000:Design
Designed by: Patrick Gerlinger Group: iGEM16_Marburg (2016-10-14)
[PanD] Aspartate 1-decarboxylase from C. glutamicum [Codon optimized]
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Seqeuencing of panD in the operon plasmid
Sequencing of panD as part of the operon plasmid that was designed as part of our project. We used primers that bind in the adjacent gene
Design Notes
While optimized for codon usage in E. coli we had to consider the biobrick restriction sites of EcoRI, PstI, XbaI, SpeI. We manually altered the sequence to avoid these cut sites.
Source
Synthesized DNA Sequence.