RNA

Part:BBa_K1963002:Experience

Designed by: Frank Sargent   Group: iGEM16_Dundee_Schools   (2016-10-07)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1963002

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iGEM Dundee_Schools 2016

Good part. The gBlock provided by IDT was perfect and the protocol provided on the main page worked after a couple of trial runs. We were able to make sRNA fusions to target expression of the fliC gene from E. coli (BBa_K1963004 and BBa_K1963010). The fliC target, encoding flagellin, was chosen as a Proof-of-Concept, since successful sRNA inhibition should affect swimming, which can be tested in the laboratory, but the flagellum is also important for pathogenesis in many bacteria.

User Reviews

UNIQ530bf6fb11733e9b-partinfo-00000003-QINU UNIQ530bf6fb11733e9b-partinfo-00000004-QINU