Coding

Part:BBa_K1963001:Design

Designed by: Frank Sargent   Group: iGEM16_Dundee_Schools   (2016-10-07)


Hfq from Serratia marcescens DB10/DB11


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are no EcoRI/XbaI/SpeI/PstI sites in this short coding sequence.


Source

This was amplified from genomic DNA.

References

Updegrove TB, Zhang A, Storz G. (2016) Hfq: the flexible RNA matchmaker.Curr Opin Microbiol 30:133-138. doi: 10.1016/j.mib.2016.02.003.

Wilf NM, Salmond GP. (2012) The stationary phase sigma factor, RpoS, regulates the production of a carbapenem antibiotic, a bioactive prodigiosin and virulence in the enterobacterial pathogen Serratia sp. ATCC 39006. Microbiology 158: 648-658. doi: 10.1099/mic.0.055780-0.

Wilf NM, Williamson NR, Ramsay JP, Poulter S, Bandyra KJ, Salmond GP. (2011) The RNA chaperone, Hfq, controls two luxR-type regulators and plays a key role in pathogenesis and production of antibiotics in Serratia sp. ATCC 39006. Environ Microbiol 13: 2649-2666. doi: 10.1111/j.1462-2920.2011.02532.x.

Seung Min Yoo, Dokyun Na & Sang Yup Lee (2013) Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature Protocols 8, 1694–1707. doi:10.1038/nprot.2013.105