Generator

Part:BBa_K1961005:Design

Designed by: Yi-Ting Lin   Group: iGEM16_HSiTAIWAN   (2016-10-03)


PbrR generator expressed by GFP under control of general promoter (J23102)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1111


Design Notes

PbrR is a Pb2+ binding protein. In addition to the PbrR generator with the PTet promoter(K1961004), we designed a PbrR generator (K1961005; also with GFP reporter) under control of general promoter(J23102). We compared their GFP expression to measure their PbrR generation.

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Source

We used parts from iGEM stocks: J23102+B0032+I721002+B0010+B0012+I721001+B0034+E1010+B0010+B0012

References