Part:BBa_K196014:Design

Final GluColi device.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2263
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1273
  Illegal AgeI site found at 636
  Illegal AgeI site found at 748
  Illegal AgeI site found at 1172
  Illegal AgeI site found at 1657
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

As many mutations were needed to make the part compatible with the standard 10, we decided to make hfsG and hfsH synthetized by [http://genearts.com/ GeneArt]. We also optimized it for E. coli. We mean that the codons were changed to favour the most present in E. coli.

Source

HfsG and HfsH sequences come from Caulobacter crescentus.