Coding

Part:BBa_K1959003:Design

Designed by: Junyu Chen   Group: iGEM16_SCAU-China   (2016-10-04)


BKT coding sequence fused with Pea transit peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1085
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 478
    Illegal SapI site found at 900


Design Notes

A pea transit peptide has been functionally fused to the BKT cds. The BKT codon has made some optimization for expression in rice. An Pst I restriction site has been mutated by site-directed mutation.


Source

Original sequence can be accessed from Genebank, Genebank accession: AY860820.1.

References