Coding
Part:BBa_K1959002:Design
Designed by: Junyu Chen Group: iGEM16_SCAU-China (2016-10-04)
Modification of β-carotene hydroxylase (BHY)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 334
Illegal NgoMIV site found at 726
Illegal NgoMIV site found at 793 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization has been made for expression in Orazy sativa and a PstI restriction site has mutated.
Source
The origin BHY sequence can be accessed from Genebank. Genebank accession: BD250390.1 .