Coding

Part:BBa_K1959001:Design

Designed by: Junyu Chen   Group: iGEM16_SCAU-China   (2016-10-03)


Modification of phytoene desaturase (PDS/CrtI)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 242
    Illegal XhoI site found at 1372
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1582
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been optimized for expression in Orazy sativa and two restriction sites have been mutated in order to fit the requirement of the Registry.


Source

The original sequence of this part can be accessed from Genebank. Genebank accession:D90087.

References