Regulatory
Part:BBa_K1955003:Design
Designed by: Justine Hsu Group: iGEM16_CGU_Taiwan (2016-10-14)
Leishmania-5 prime UTR-Hygromycin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The hemagglutinin (HA) part is a 1700 bp DNA sequence acquired from GenBank (Accession# J02176.1). Some nucleotides of HA sequence had been changed to avoid the restriction sites. In order to compare the protein expression efficiency of our expression vector and the original pX63HYG shuttle vector, we add BamHI restriction site at the head and tail of HA coding sequence for the ligation of pX63HYG shuttle vector and HA sequence.
Source
The whole sequence of Hemagglutinin (HA) from H1N1 is acquired from GenBank.