Coding
C-IgAP

Part:BBa_K195410:Design

Designed by: Syue-Liang Lin   Group: iGEM09_NYMU-Taipei   (2009-10-21)


C-terminal of IgA protease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 121
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 132
    Illegal NgoMIV site found at 757
    Illegal AgeI site found at 532
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Design Notes

C-IgAP(BBa_J32018) is exist part,but this part is not exist in plate. We need to PCR this sequence from Neisseria gonorrhoeae by ourself. There have some problem:Pst1 cutting site...So we have to mutate these site dierectly.



Source

Neisseria gonorrhoeae FA1090

References