Composite

Part:BBa_K1952009

Designed by: Jessica Zhao   Group: iGEM16_Kingsborough_NY   (2016-10-19)


Hydrazine Synthase subunit operon

Lac-promoter driven operon, with three separate open reading frames representing K. stuttgartiensis Hydrazine Synthase multi-subunit enzyme. (Subunits gamma, beta, and alpha, respectively.)

Expression or activity not yet confirmed.

This enzyme is a key step in the anaerobic ammonia oxidation pathway, which converts ammonia (NH4) and nitric oxide (NO) into nitrogen gas (N2). The Hydrazine Synthase enzyme takes the two starting reactants and produces the intermediate hydrazine (N2H4).

For more information see Kartal, B., Keltjens, J., (2016) Anammox Biochemistry: A Tale of Two Heme c proteins. Trends in Biochemical Sciences. [http://dx.doi.org/10.1016/j.tibs.2016.08.015]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2297
    Illegal BamHI site found at 966
    Illegal BamHI site found at 1766
    Illegal BamHI site found at 1967
    Illegal BamHI site found at 2090
    Illegal BamHI site found at 4528
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3583
    Illegal AgeI site found at 2434
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1443


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Categories
Parameters
None