Coding

Part:BBa_K1946000:Design

Designed by: Musa Efe Işılak   Group: iGEM16_Bilkent-UNAMBG   (2016-10-13)


ChnR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 514


Design Notes

Codon optimized for E. Coli k12


Source

The source of this part is amino acid sequence from GenBank: BAA86295.1

References

Iwaki H, Hasegawa Y, Teraoka M, Tokuyama T, Bergeron H, Lau PC. Identification of a transcriptional activator (ChnR) and a 6-oxohexanoate dehydrogenase (ChnE) in the cyclohexanol catabolic pathway in Acinetobacter sp. Strain NCIMB 9871 and localization of the genes that encode them. Appl Environ Microbiol. 1999;65(11):5158-62.

Steigedal, Magnus and Svein Valla. "The Acinetobacter Sp. Chnb Promoter Together With Its Cognate Positive Regulator Chnr Is An Attractive New Candidate For Metabolic Engineering Applications In Bacteria". Metabolic Engineering 10.2 (2008): 121-129. Web.