Reporter

Part:BBa_K1943014

Designed by: Shixin Lu, Shiqiang Tang   Group: iGEM16_SUSTech_Shenzhen   (2016-10-13)


Bleo

In this project, we chose cho-k1 (Chinese hamster ovary) cell as our experimental object for its ability of suspended growth. We used several antibiotic resistance genes to select the successfully transfected cell. Some of them are very useful but no team has submitted related part before. We kindly hope these resistance gene can help the following team researching on mammalian cell.

The gene bleo is the resistance gene to antibiotic phleomycin D1, a kind of bleomycin. It is primarily used in selection of eukaryotic cell lines. In our project we use the concentration of 400μg/mL.

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figure: structure of zeocin,structural information taken from SciFinder for CAS 11031-11-1, stereochemical information taken from PubChem CID 72511


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 270
    Illegal NgoMIV site found at 331
  • 1000
    COMPATIBLE WITH RFC[1000]


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