Coding

Part:BBa_K1943012:Design

Designed by: Shixin Lu, Shiqiang Tang   Group: iGEM16_SUSTech_Shenzhen   (2016-09-09)


msfGFP coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


Design Notes

In our lab, we usually use sfGFP as a report for molecular experiment. In the plasmid, there is a sfGFP coding device which contains promoter BBa_J23116 and RBS BBa_B0034. Some of our backbones contain KpnI site, and we need a single KpnI site, while sfGFP contains a KpnI site. So we do site-directed mutagenesis of it. And then we design primer and do PCR of this msfGFP coding sequence and ligate it to pSB1C3 backbone.

Source

sfGFP coding sequence was from our instructor, Huangwei's lab.

References

  1. Parts Registry Assembly Help
  2. Parts Registry Transformation Guideline