Reporter

Part:BBa_K1943003:Experience

Designed by: Shixin Lu, Shiqiang Tang   Group: iGEM16_SUSTech_Shenzhen   (2016-08-29)

Applications of BBa_K1943003

After we constructed this part, we transformed it into BL21 to test the expression efficiency. Meanwhile, we did a comparison with another part only whose promoter+RBS combination differs.

Expression test of BBa_K1943003

We spread the plate for DH5α at 1:20am on 14th, Oct. The plate contains chloramphenicol. Following photo were taken every several hours after placing the plates into a 37℃ incubator. K1943003 is in the bottom-right corner in each photo.

At 12:24 (14th,Oct.)

6 7@1224.jpeg

At 14:20 (14th,Oct.)

6 7@1420.jpeg

At 18:30 (14th,Oct.)

6 7@1830.jpeg

At 20:21 (14th,Oct.)

6 7@2021.jpeg

At 22:40 (14th,Oct.)

6 7@2240.jpeg

At 8:50 (15th,Oct.)

6 7@850.jpeg

At 14:28 (15th,Oct.)

6 7@1428.jpeg

User Reviews

UNIQ1fcc16d0c77cf8f6-partinfo-00000000-QINU UNIQ1fcc16d0c77cf8f6-partinfo-00000001-QINU