RNA

Part:BBa_K1941006:Design

Designed by: Dimitri Coukos   Group: iGEM16_EPFL   (2016-10-13)


sgRNA for c6_TDH3 (1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 145
    Illegal NgoMIV site found at 174
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This version of the sgRNAs c6_TDH3 is the closest gRNA possible to the transcription start site of the TDH3 promoter. It should therefore perform the best repression via CRISPRi when in complex with dCas9 as it will avoid the binding of the RNA polymerase.

Source

some source!

References