Composite

Part:BBa_K1939004:Design

Designed by: Kyler Herrington   Group: iGEM16_UGA-Georgia   (2016-10-07)


Mutant RBS with mCherry reporter gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is one of a number of random point mutation in the 12 base pair region beginning with the RBS and ending after the first nucleotide of the start codon. This was considered when designing the PCR primers.


Source

This is a mutant of the RBS region of the native strain, S001, of M. maripaludis

References