Composite

Part:BBa_K1935013:Design

Designed by: Emilie Gounin   Group: iGEM16_Bordeaux   (2016-10-13)


White Collar Complex under control of C.elegans's promoter (pUNC119::WC1::P2A::WC2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4229
    Illegal BglII site found at 4865
    Illegal BamHI site found at 4510
    Illegal BamHI site found at 5669
    Illegal BamHI site found at 5705
    Illegal BamHI site found at 6479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6895
    Illegal NgoMIV site found at 6954
    Illegal AgeI site found at 6501
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3792
    Illegal BsaI site found at 4136
    Illegal BsaI site found at 4589
    Illegal BsaI site found at 6046
    Illegal BsaI.rc site found at 6414
    Illegal SapI.rc site found at 4731
    Illegal SapI.rc site found at 5030
    Illegal SapI.rc site found at 6296


Design Notes

Nothing relevant


Source

PROMOTEROME database : http://worfdb.dfci.harvard.edu/promoteromedb/searchPromoterome.pl?by=name&sid=UNC-119

http://www.uniprot.org/uniprot/F8MUC4

https://www.ncbi.nlm.nih.gov/nucleotide/698997583?report=genbank&log$=nuclalign&blast_rank=3&RID=ZYZ7SCEW014



References