Regulatory

Part:BBa_K1935009:Design

Designed by: Jean DESCARPENTRIE   Group: iGEM16_Bordeaux   (2016-10-13)


pMYO-3 : Specific muscle promoter of C.elegans


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 53
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1446


Design Notes

Mutagenesis was done to abolish three illegal restriction sites : SpeI (1018-1023), PstI (1212-1217) and XbaI (2025-2030).



Source

PROMOTEROME : http://worfdb.dfci.harvard.edu/promoteromedb/searchPromoterome.pl?by=name&sid=myo-3

References

http://www.wormbase.org/species/c_elegans/gene/WBGene00003515#01-9g-3