Coding

Part:BBa_K1935005:Design

Designed by: Emilie Gounin   Group: iGEM16_Bordeaux   (2016-10-13)


White collar-2 (WC-2) - photoreceptor in Neurospora tetrasperma


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 25
    Illegal BamHI site found at 61
    Illegal BamHI site found at 835
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1251
    Illegal NgoMIV site found at 1310
    Illegal AgeI site found at 857
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 402
    Illegal BsaI.rc site found at 770
    Illegal SapI.rc site found at 652


Design Notes

A mutagenesis was done to abolish an illegal restriction site (Pst1 : 425 to 430 pb, CTGCAG => CAGCAG)


Source

https://www.ncbi.nlm.nih.gov/nucleotide/698997583?report=genbank&log$=nuclalign&blast_rank=3&RID=ZYZ7SCEW014

References